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更新時(shí)間:2024-07-16      
瀏覽次數(shù):910Cedarlane 淋巴細(xì)胞分離柱
Cedarlane公司成立于加拿大,27年間,已經(jīng)逐步發(fā)展成為生命科學(xué)行業(yè)的一家跨國(guó)公司,在加拿大和美國(guó)擁有超過(guò)100名員工。
Cedarlane公司的研究領(lǐng)域。
公司為生命科學(xué)研究人員和臨床醫(yī)生提供無(wú)數(shù)的生物和生化產(chǎn)品,提供來(lái)自幾乎所有世jie上zui 知 ming 的國(guó)際制造商的產(chǎn)品。交貨及時(shí),所有產(chǎn)品都在適當(dāng)?shù)乃铚囟认聝?chǔ)存、接收和運(yùn)輸。員工與客戶和供應(yīng)商密切合作,提供個(gè)性化和全面的體驗(yàn),以反映客戶至上的核心價(jià)值。通過(guò)提供全球超過(guò) 600 萬(wàn)種試劑盒和試劑的門戶,客戶擁有貨運(yùn)整合的優(yōu)勢(shì)以及其中固有的便利性和成本節(jié)約;Cedarlane 是一站式商店。
主要分銷產(chǎn)品線包括:抗體、細(xì)胞培養(yǎng)基和過(guò)濾、原代細(xì)胞和細(xì)胞系、蛋白質(zhì)和肽、酶和抑制劑、成像和顯微鏡資源、檢測(cè)/ELISA試劑盒、精細(xì)化學(xué)品和生化、DNA/RNA分離和純化。
主要制造產(chǎn)品包括:?jiǎn)慰寺『投嗫寺】贵w、細(xì)胞分離培養(yǎng)基、組織分型補(bǔ)體和高度表征的神經(jīng)元細(xì)胞系。
Lympholyte®-H是一種密度梯度分離介質(zhì),專門設(shè)計(jì)用于從人外周血和臍帶血中分離淋巴細(xì)胞和單核細(xì)胞。
注意:使用前充分搖晃混勻,靜止2-3min,待氣泡消失后試用。(室溫條件下使用分離液,分離液密度會(huì)隨溫度的變化而發(fā)生改變)
樣本制備可使用無(wú)血清培養(yǎng)基,減少樣本粘度,更利于分離。
操作步驟:
1.在含有抗凝血?jiǎng)┑脑嚬苤惺占搜?/span>
2.用等量培養(yǎng)基1:1稀釋血液;
3.根據(jù)不同的方法進(jìn)行分離:
①在離心管中加入3 mL Lympholyte®-H;
②使用移液管,小心地將6 mL稀釋的血液鋪在Lympholyte®-H上,在液面上盡可能少地混合(圖A);
③由于Lympholyte®-H的密度比細(xì)胞懸液更大,因此將形成一個(gè)明顯的界面(圖C)。
方法B:
①在離心管中加入6 mL稀釋后的血液。將一個(gè)大的(23厘米)移液管放置到試管的底部(圖B);
②慢慢地向移液管中加入Lympholyte®-H,重力使其在稀釋的血液下分層;
③繼續(xù)處理,直到3mL Lympholyte®-H在稀釋的血液下分層。由于Lympholyte®-H比細(xì)胞懸液密度更大,因此會(huì)形成明顯的界面(圖C);
4.在室溫下,以800g離心20分鐘;
5.離心后,界面上將有一個(gè)明顯的淋巴細(xì)胞層(圖D)。使用移液管,小心地將細(xì)胞吸出,并轉(zhuǎn)移到一個(gè)新的離心管中;
6.用培養(yǎng)基稀釋轉(zhuǎn)移的細(xì)胞,以降低溶液的密度。800g離心10分鐘,沉淀淋巴細(xì)胞,然后棄用上清液;
Note:如果需要去除血小板,可在這一步進(jìn)行操作:
方法A
A.加入0.5 mL緩沖液和50 uL凝血酶,然后輕輕重懸細(xì)胞;
B.用緩沖液補(bǔ)足10mL,并充分混合;
C.非常緩慢地(100g)離心細(xì)胞3分鐘;血小板會(huì)聚集并沉淀在底部;
D. 取出上清液(包含細(xì)胞),放入另一個(gè)試管中,留下聚集的血小板。
方法B
A.800 g離心1分鐘,沉淀細(xì)胞;
B.倒出上清液,用緩沖液重懸細(xì)胞,重復(fù)2次;
C.此時(shí)會(huì)觀察到,當(dāng)血小板被移除時(shí),產(chǎn)生的上清液會(huì)變得更加清晰。
7.在進(jìn)一步處理之前,在培養(yǎng)基中清洗淋巴細(xì)胞2-3次(在這個(gè)階段可以使用含有血清的培養(yǎng)基)。

以下是Cedarlane公司的熱銷產(chǎn)品
產(chǎn)品名稱  | 貨號(hào)  | 規(guī)格  | 
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid  | CL5020  | 500ml  | 
Baby Rabbit Complement, Tissue Culture Grade - Frozen  | CL3441-S  | 1ml  | 
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid  | CL5016  | 6x100mL  | 
Low-Tox® Guinea Pig Complement, Lyophilized  | CL4051  | 5x1ml  | 
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid  | CL5031  | 100ml  | 
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid  | CL5115  | 100ml  | 
Low-Tox®-M Rabbit Complement, Lyophilized  | CL3051  | 5x1ml  | 
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid  | CL5030  | 5x30ml  | 
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid  | CL5015  | 100ml  | 
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid  | CL5026  | 6x500ml  | 
Anti-Mouse Asialo GM1, Ig Fraction (Polyclonal)(rabbit Ig)  | CL8955  | 1 mL  | 
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid  | CL5120  | 500ml  | 
Human Complement, Pooled, Frozen  | CL6600-1  | 1 ml  | 
Anti-Mouse/Human Mac-2 (Galectin-3), Purified (Clone M3/38) (rat IgG2a)  | CL8942AP  | 100ug  | 
Anti-Human Tamm Horsfall Purifed (Clone 10.32) (mouse IgG2b)  | CL1032AP  | 250ug  | 
Anti-Mouse Complement Component C3, FITC (Clone RmC11H9) (rat IgG2a)  | CL7503F  | 100ug  | 
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid  | CL5110  | 5x30ml  | 
Lympholyte®-1.1, sterile liquid  | CL6155  | 500mL  | 
Baby Rabbit Complement, Lyophilized  | CL3441  | 10x1ml  | 
Anti-Human Factor VIII:C HRPO (Polyclonal) (sheep IgG)  | CL20035HP  | 0.2 mg  | 
Anti-Human Factor VIII:C Aff.Pur.HRPO(Paired Abs)  | CL20035K  | 4 plates  | 
Low-Tox®-H Rabbit Complement, Lyophilized  | CL3331  | 10x1mL  | 
Chicken Gamma Globulin (purified antigen)  | CLF802-1  | 50mg  | 
Lympholyte®-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid  | CL5041  | 100mL  | 
Lympholyte®-Rabbit Cell Separation Media (Isolation of lymphocytes from Rabbit lymphoid tissue), sterile liquid  | CL5050  | 5x30ml  | 
Standard Rabbit Complement, Lyophilized  | CL3111  | 25x1ml  | 
Anti-Rat CD90 (Thy 1.1) Ascites (Clone OX-7) (mouse IgG1)  | CL005A  | 0.5ml  | 
Rabbit Complement MA, Lyophilized  | CL3221  | 10x1ml  | 
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid  | CL5070  | 100mL  | 
Anti-Human Fibrinogen Aff.Pur./HRPO (Paired Abs)  | CL20067K  | 5 plates  | 
Lympholyte®-1.1, sterile liquid  | CL5095  | 500mL  | 
O-sialoglycoprotein endopeptidase, Lyophilized  | CLE100  | 1.2mg  | 
Anti-Mouse Thrombocyte (platelet), Antiserum, (Polyclonal) (rabbit serum)  | CLA31440  | 5mL  | 
Anti-Human Tissue Plasminogen Activator, Affinity Purified (Polyclonal) (sheep IgG)  | CL20115AP  | 0.5 mg  | 
Anti-Human Factor IX Aff.Pur./HRPO (Paired Abs)  | CL20041K  | 5 plates  | 
Baby Rabbit Complement, Tissue Culture Grade, Frozen  | CL3441-S100  | 100ml  | 
Standard Guinea Pig Complement, Lyophilized  | CL5000  | 5x5ml  | 
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid  | CL5010  | 5x30mL  | 
Human Interleukin-17A  | CL101-17-25UG  | 25ug  | 
Anti-Mouse Collagen Type I, purified IgG  | CL50151AP-1  | 0.1ml  | 
Anti-Mouse CD25 , Purified (Clone PC61.5.3) (rat IgG1)  | CL8925AP  | 250ug  | 
Anti-Rat IL-10, Purified, (Polyclonal) (rabbit IgG)  | CL9583AP  | 50ug  | 
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid  | CL5071  | 250 mL  | 
Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit serum)  | CLA31140  | 5mL  | 
Anti-Rat Myeloid Lineage, FITC (Clone OX82) (mouse IgG1)  | CL081F  | 100 ug  | 
Anti-Rat Macrophage, Adsorbed Antiserum, (Polyclonal) (rabbit serum)  | CLAD51240  | 2mL  | 
Baby Rabbit Complement- Tissue Culture Grade, Frozen  | CL3441-S50  | 50 ml  | 
Recombinant Human Gastrokine 3  | CLPRO2033-2  | 10ug  | 
Anti-Rat CD45RA/B FITC (Clone OX-33) (mouse IgG1)  | CL033F  | 100ug  | 
Mini Super PAP PEN (tip 1/10" diameter, 3 mL)  | MU22  | EA  | 
Anti-Rat CD11b/c Purified (Clone OX-42) (mouse IgG2a)  | CL042AP  | 250ug  | 
Anti-Human Factor H (FITC) (Clone: OX-24) (mouse IgG1)  | CL7672F  | 100 ug  | 
Anti-Mouse I-Ad, Ascites (Clone 34-5-3S) (mouse IgG2a)  | CL8713A  | 0.5 mL  | 
Anti-Human Antithrombin III Affinity Purified (Polyclonal) (sheep IgG)  | CL20015AP  | 0.5mg  | 
Cedarlane/Anti-Rat CD45, 10 nm Gold Conjugate,OD3 (Clone OX-1) (mouse lgG1)  | CL009G  | 0.5 mL  | 
Cedarlane/Rat IL-2ELISA kit(precoated)  | CL00113K-96  | 96T  | 
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